Fig. 7

c-MYC promotes the transcriptional expression of EFTUD2. A Venn diagram displays the intersection of target genes downregulated by c-MYC knockdown in HCT116 cells with those upregulated, as shown in Fig. S1B. B and C Scatterplots illustrate the correlation between EFTUD2 and c-MYC in the TCGA cohort. D-F Feature plots (D and E) and a scatterplot (F) depict the correlation between EFTUD2 and c-MYC in the TCGA-CRC single cell transcriptome. G Analysis using Cistrome Data Browser and JASPAR database show the predicted c-MYC binding motifs in the EFTUD2 promoter. H Schematic illustrates the top predicted c-MYC binding site within the EFTUD2 promoter region. I ChIP-qPCR analysis show c-MYC directly binding to the specific site on the EFTUD2 promoter in SW480 and HCT116 cells. J Dual luciferase reporter assay shows luciferase activity controlled by EFTUD2 wild-type (Luc-WT) but not the mutant (Luc-Mut) promoter in a c-MYC dependent manner. Each bar represents the mean values ± SD, *P < 0.05; **P < 0.01; ***P < 0.001