Fig. 8

The effect of EFTUD2 on attenuating the chemotherapy efficacy of 5-FU is dependent on c-MYC stabilization. A and B Western blotting analysis of changes in EFTUD2 protein levels before and after modulation of c-MYC expression in SW480 (A), HCT116, and Caco-2 (B) cells treated with 5-FU. C and D Clone formation (C) and EdU (D) assays showing the influence of EFTUD2 upregulation on cell survival and proliferation before and after c-MYC knockdown in SW480 cells treated with 5-FU. E and F Clone formation (E) and EdU (F) assays showing the influence of EFTUD2 downregulation on cell survival and proliferation before and after c-MYC overexpression in HCT116 and Caco-2 cells treated with 5-FU. Each bar represents the mean values ± SD, *P < 0.05; **P < 0.01; ***P < 0.001