Fig. 8

Hypoxia regulates the lncRNA AC016727.1/BACH1/HIF-1α signaling loop. a The recognition interaction motif of HIF-1α and lncRNA A016727.1 was predicted using the JSAPAR database. b mRNA expression of lncRNA AC016727.1, HIF-1α, and BACH1 in five different NSCLC cell subtypes (A549, H1299, H23, H266, and H1703 cells) under normoxic and hypoxic conditions. c HIF-1α expression was inhibited in A549 and H1299 cells following lncRNA AC016727.1 knockdown under aerobic conditions. Quantitative analysis is presented on the right. d HIF-1α expression was promoted in H226 and H1703 cells after lncRNA AC016727.1 overexpression under aerobic conditions. On the right, quantitative analysis is shown. e–f Expression of HIF-1α, BACH1, and lncRNA AC016727.1 after HIF-1α knockdown in A549 and H1299 cells under normoxic and hypoxic conditions. g Hypoxia-sensing elements in the lncRNA AC016727.1 promoter. Binding of lncRNA AC016727.1 and HIF-1α was validated using the luciferase reporter assay. h Under normoxic and hypoxic conditions, a ChIP assay was performed to verify the binding between the HRE of the lncRNA AC016727.1 promoter and HIF-1ɑ. i-j Expression of HIF-1α, BACH1, and lncRNA AC016727.1 after lncRNA AC016727.1 knockdown in A549 and H1299 cells under normoxic and hypoxic conditions. k-l Expression of HIF-1α, BACH1, and lncRNA AC016727.1 after lncRNA AC016727.1 overexpression in H226 and H1703 cells under normoxic and hypoxic conditions. m–n Expression of HIF-1α, BACH1, and lncRNA AC016727.1 following BACH1 knockdown in H226 and H1703 cells under normoxic and hypoxic conditions