Fig. 2

ABCB1 overexpression in PDAC PR cell lines. A Venn diagrams showing the up-regulated mRNAs (upper panel) or proteins (lower panel) in Patu-T PR and Suit-2.028 PR compared to the respective CTR. A list of the genes in common for each dataset can be found at Supplemental Table S3. B Venn diagram showing targets upregulated in both RNA-seq (Patu-T PR in green and Suit-2.028 in red) and proteomics analysis (Patu-T PR in blue and Suit-2.028 in yellow) in Patu-T PR and Suit-2.028 PR compared to the respective CTR. C Relative gene expression of ABCB1 in CTR (blue), GR (green) and PR (red), measured by RT-qPCR. D Western blot analysis of ABCB1 expression and β-actin (B-act) as loading control in the indicated CTR, GR, and PR cell models. Uncropped Western blot membranes can be found in Figure S4. E Representative confocal images of PDAC cell lines stained with 1 μg/mL of Hoechst33342 for 2 h at 37 °C in growth medium. Scale bar: 100 μm. F ABCB1 expression levels assessed by immunohistochemistry (IHC) in surgical specimens from PDAC patients who were subsequently treated with gemcitabine/nab-paclitaxel as first-line therapy. Three specimens with low and three specimens with high expression levels are shown. Scale bar: 200 μm