Table 2 Primer sequences used in qRT-PCR
From: Unveiling the role of osteosarcoma-derived secretome in premetastatic lung remodelling
Gene | Forward (5’ – 3’) | Reverse (5’ – 3’) |
|---|---|---|
CCL2 | CAAGATGATCCCAATGAGTAG | TTGGTGACAAAAACTACAGC |
CXCL2 | GGGTTGACTTCAAGAACATC | CCTTGCCTTTGTTCAGTATC |
CXCR2 | CTACTGCAGGATTAAGTTTACC | GACGTATATTACAACCACAGC |
FN1 | CCTATAGGATTGGAGACACG | GTTGGTAAATAGCTGTTCGG |
GAPDH | GCCTTCCGTGGTCCTACC | GCCTGCTTCACCACCTTC |
HPRT1 | GTTGAAGATATAATTGACACT | GGCATATCCAACAACAAA |
IL-6 | TTCCATCCAGTTGCCTTC | TTCTCATTTCCACGATTTCC |
IL-1β | TCTATACCTGTCCTGTGTAATG | GCTTGTGCTCTGCTTGTG |
PRG4 | GATAATGCTATTCCAGGCAC | CATCCAGAAATAATGACCTCG |
S100A8 | ATACAAGGAAATCACCATGC | ATATTCTGCACAAACTGAGG |
S100A9 | CTTTAGCCTTGAGCAAGAAG | TCCTTCCTAGAGTATTGATGG |
TGF-β | ATGGTGGACCGCAACAAC | TTGCTATATTTCTGGTAGAGTTCC |
TNF-α | CAAGGGACTAGACAGGAG | TGCCTCTTCTGCCAGTTC |