Fig. 2

SNHG17 in THP-1 cell-derived TAMs enhanced anaerobic glycolysis and promoted M2 polarization and malignant progression of PCs. A Schematic representation of induction of THP-1 into TAMs in vitro. B The mRNA expression of M2 markers (CD206, CD163, IL6, IL10, TGFB and Arg1) and M1 markers (IL1B and CD80) after knockdown of SNHG17 in THP-1 derived TAMs. C Flow Cytometry ananlysis of CD80/CD86/CD163/CD206 expression in THP-1 derived TAMs (shNC, sh1-SNHG17 and sh2-SNHG17). D-E CCK8 analysis of PANC1 co-cultured with THP-1 derived TAMs (D) and PATU-8988 co-cultured with THP-1 derived TAMs (E). F-G CTG analysis of two patient-derived organoids (PDO1# and PDO2#). (H) Representative colony formation images of PANC1 co-cultured with THP-1 derived TAMs (shNC, sh1-SNHG17 and sh2-SNHG17) and PATU-8988 co-cultured with THP-1 derived TAMs (shNC, sh1-SNHG17 and sh2-SNHG17). I-J Colony numbers of PANC-1 and PATU-8988 in each group. K-L Representative migration (K) and invasion (L) images of PANC1 co-cultured with THP-1 derived TAMs (shNC, sh1-SNHG17 and sh2-SNHG17) and PATU-8988 co-cultured with THP-1 derived TAMs (shNC, sh1-SNHG17 and sh2-SNHG17). M–N Migrated and invaded PANC-1cells (M) per field and PATU-8988 cells (N) per field in each group (shNC, sh1-SNHG17 and sh2-SNHG17). O-Q Glucose uptaking (O) and Lactic acid concentration (P, Q) analysis of THP-1 derived TAMs in each group (shNC, sh1-SNHG17 and sh2-SNHG17). R-S Seahorse analysis of THP-1 cells (shNC, sh1-SNHG17 and sh2-SNHG17) co-cultured with PANC-1 cells. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001