Fig. 3

SNHG17 in THP-1 cell-derived TAMs boosted growth and metastasis of PCs in vivo. A Images of BALB/c nude mice which were injected with THP-1 cells (shNC and sh1-SNHG17) and PANC-1 cells. B Weights of subcutaneous tumors from BALB/c nude mice in each group. (n = 6) (C) and (F) K-M survival curves for BALB/c nude mice from the subcutaneous tumors mouse model (C) and liver metastasis mouse model (F) in the shNC and sh1-SNHG17 groups. D Images of livers from BALB/c nude mice co-injected with THP-1 cells and PANC-1 cells in the spleen. D and G Protein expression levels (PGK1, GLUT1, ENO1, HK2, and LDHA) in macrophages isolated from subcutaneous tumors (D) and metastatic lesions (G). (n = 4) (H) and (N) HE stained images of tissues from subcutaneous tumors mouse model (H) and liver metastasis mouse model (N) left: scale bar = 400 μm; right: scale bar = 100 μm. (I-L) and (O-R) IHC staining images of Ki-67 (I, O), CD68 (J, P), CD163 (K, Q), and PGK1(L, R) in tissues from the subcutaneous tumors mouse model and liver metastasis mouse model. left: scale bar = 400 μm; right: scale bar = 100 μm. (M) and (S) IHC score (Ki-67, CD68, CD163, and PGK1) of tissues from the subcutaneous tumors mouse model (M) and liver metastasis mouse model (S) in the shNC and sh1-SNHG17 groups. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001