Fig. 4

SNHG17 sponged miR-628-5p to release PGK1 mRNA. A FISH visualization of SNHG17 (red) in THP-1 cells following co-culture with either PATU-8988 or PANC-1 cells. Nucleus was stained with DAPI (blue). Scale bar: 20 μm. B-C Representative FISH images were analyzed to determine the intensity values for SNHG17 and DAPI in THP-1 cells-derived TAMs. Red represents SNHG17. Blue represents DAPI. D Relative expression levels of SNHG17 in the cytoplasm and nucleus of THP-1 cell-derived TAMs. E The RNA expression level of miR-628-5p in THP-1 cell-derived TAMs (shNC, sh1-SNHG17, and sh2-SNHG17). F-G Expression level of pre-miR-628-5p (F) and pri-miR-628-5p (G) in THP-1 cell-derived TAMs transfected with negative control plasmids (pcDNA) or SNHG17 plasmids. H Dual-luciferase assay was used to assess the ability of miR-628-5p to bind to the SNHG17 promoter region. I RIP analysis of THP-1 cell-derived TAMs indicated that AGO2 could interact with SNHG17 and miR-628-5p. J-K Dual-luciferase activity in HEK-293 T cells post-cotransfection with SNHG17 (WT or MUT) and miR-628-5p mimics. L Schematic representation of the screening process for downstream target genes of miR-628-5p. M The expression level of SNHG17 in THP-1 cell-derived TAMs transfected with miR-628-5p inhibitor or miR-628-5p mimics. N–O Dual-luciferase activity in HEK-293 T cells post-cotransfection with PGK1 (WT or MUT) and miR-628-5p mimics. P The protein expression level of PGK1, GLUT1, ENO1, HK2, and LDHA in THP-1 cell-derived TAMs (co-cultured with either PANC-1 cells or PATU-8988 cells) transfected with negative control, sh1-SNHG17, or sh2-SNHG17 plasmids. Q WB assays indicated that miR-628-5p could reverse the SNHG17-induced promotion of aerobic glycolysis in THP-1 cell-derived TAMs. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001