Fig. 1

RIT1 is closely associated with the mitotic processes in HCC. A Gene set enrichment analysis (GSEA) of a TCGA LIHC cohort with 374 tissues stratified by the mean cut-off value of RIT1 expression. B GO, KEGG, and HALLMARK enrichment analysis based on differential genes detected by RNA-Seq in the RIT1 overexpressing and control group of HCC cells. The terms are ordered by -log10 FDR. C Comparison of the representative time-lapse images of dynamic division at different time points between RIT1 knockdown and control groups in MHCC-97H and HCC-LY10 cells (upper section). The quantitation histogram showing the percentage of abnormal division cells (lower section). Data are presented as mean ± SD of three independent experiments. *** P < 0.001. The P values were calculated by unpaired Student’s t-test in (C)