Fig. 2

Knockdown of RIT1 induces mitotic catastrophe and apoptosis in HCC cells. A, B Representative immunofluorescence staining images of α-tubulin (red) and DAPI (blue) show spindle morphology during mitosis in MHCC-97H (A) and HCC-LY10 cells (B) with RIT1 knockdown and control (left). The quantitation histogram showing the percentage of cells with multipolar spindle (right). C, D Representative immunofluorescence staining images of α-tubulin (red) and DAPI (blue) show nucleus morphology in MHCC-97H (C) and HCC-LY10 cells (D) with RIT1 knockdown and controls (left). The quantitation histogram showing the percentage of cells with multinuclear (right). Cells were enriched in mitosis utilizing nocodazole. Scale bars, 20 μm. E Flow cytometry analysis of apoptosis in MHCC-97H and HCC-LY10 cells with RIT1 knockdown and control group (left). The quantitation histogram showing the percentage apoptotic cells (right). Data are presented as mean ± SD of three independent experiments. *** P < 0.001. The P values were calculated by unpaired Student’s t-test