Fig. 6

RIT1 affects the acetylation of SMC3 during mitosis. A, B Western blot analysis of acetylated-SMC3, total SMC3, and RIT1 protein levels during different cell phases in MHCC-97H (A) and HCC-LY10 cells (B) with RIT1 knockdown. C, D Western blot analysis of acetylated-SMC3, total SMC3, and RIT1 protein levels during different cell phases in Huh7 (C) and Hep3B (D) cells with RIT1 overexpression. Cells were enriched in different cycle phases by releasing from the TT block for the indicated time. E qPCR assays of ESCO1 mRNA expression following si-ESCO1 transfection in RIT1-overexpressed Huh7 and Hep3B cells. F The cell proliferation ability was examined using CCK8 assay in RIT1-overexpressing Huh7 and Hep3B cells with ESCO1 knockdown. Data are presented as mean ± SD of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001. The P values were calculated by unpaired Student’s t-test in (E, F)