Fig. 7

RIT1 promotes HCC proliferation by binding to PDS5 to protect the acetylation of SMC3. A Co-IP analysis of RIT1 and PDS5 in Huh7 and Hep3B cells with RIT1 overexpression. B Western blot analysis of acetylated-SMC3, total SMC3, PDS5A/B, and RIT1 protein upon knockdown of PDS5A/5B in RIT1-overexpressing Huh7 and Hep3B cells. C The cell proliferation ability was examined using CCK8 assay upon knockdown of PDS5A/5B in RIT1-overexpressed Huh7 and Hep3B cells. Data are presented as mean ± SD of three independent experiments. * P < 0.05, ** P < 0.01. The P values were calculated by unpaired Student’s t-test in (C)