Fig. 1

The effect of Gli1 in PDAC cells. A Fluorescence expression of lentivirus transfection (200 ×). B Protein levels of Gli1 were measured by Western blotting. C qRT-PCR was used to assess relative expression levels of Gli1. D OD450 values were measured with a CCK-8 kit. E The number of invasive cells is shown by crystal violet staining. F Protein levels of Ki-67, E-cadherin, and Vimentin were measured by Western blotting. *P < 0.05 for lenti-Gli1 vs. EV-Gli1