Fig. 6

The verification of mechanism of exosomal circ-0011536 as ceRNA and competitive binding of miRNA-451a. A qRT-PCR was used to assess relative expression levels of VGF. *P < 0.05 for si-VGF vs. si-VGF NC. B, D Protein levels of VGF were measured by Western blotting. C qRT-PCR was used to assess relative expression levels of VGF. E, F The axon length of DRG (200 ×). *P < 0.05 for lenti-Gli1 exos/OE-circ + miR-451a mimics vs. lenti-Gli1 exosomes; ^#P < 0.05 for lenti-Gli1 exosomes/OE-circ + si-VGF vs. lenti-Gli1 exosomes. G Sequences of wild-type and mutant mmu-circ-0001261 and VGF that disrupt interaction with miR-451a are shown. The relative dual luciferase activity isindicated. *P < 0.05 for miR-451a vs. miR-NC