Fig. 1

Proportions of T-cell subsets in the tumor microenvironment are independent of tumor histological types. A Freshly resected tumors from various histologies (n = 72) were collected and dissociated into a cell suspension and stained for T cell subset identification. Immune checkpoints (ICPs) expression was assessed in CD8⁺, CD4⁺FoxP3⁻ and CD4⁺FoxP3⁺ T cells at the protein level using flow cytometry (n = 35) and at the transcriptomic level using single-cell RNA sequencing, including TCR sequencing (n = 5). Created with BioRender.com. Flow cytometry analysis from 72 fresh tumor specimens. B Percentage of CD45⁺ among live cells in the different histologies. C Percentage of CD3⁺ T-cells among CD45⁺ cells according to the different histologies. Percentage of CD8⁺ (D), CD4⁺FoxP3⁻ (E) and CD4⁺FoxP3⁺ (F) among CD3⁺ cells in the different histologies. The red dotted line delineates the median of the whole cohort. Dunn’s multiple comparison test, *p value ≤ 0.05; **p value ≤ 0.01; ***p value ≤ 0.001; ****p value ≤ 0.0001. MM: Metastatic Melanoma; NSCLC: Non-Small Cell Lung Carcinoma; RCC: Renal Cell Carcinoma; HNSCC: Head and Neck Squamous Cell Carcinoma; EOC: Epithelial Ovarian Cancer; UC: Urothelial Carcinoma