Fig. 2
Contact with PC cells reshapes the transcriptional profile of endothelial cells. A Fold differences of mRNAs of angiogenesis-related genes, between HUVEC (yellow bars) or HAEC (red bars) cocultured with wild type DU145 versus HUVEC or HAEC, respectively. A significant threshold of a twofold change in gene expression corresponded to p < 0.001. Experiments were performed in duplicate. B Fold differences of mRNAs of angiogenesis-related genes between HUVEC (yellow bars) or HAEC (red bars) cocultured with wild type PC3 versus HUVEC or HAEC, respectively. A significant threshold of a twofold change in gene expression corresponded to p < 0.001. Experiments were performed in duplicate. C Fold differences of mRNAs of angiogenesis-related genes between HUVEC cocultured with wild type DU145 (yellow bars), or PC3 (green bars), versus HUVEC alone, and between HAEC cocultured with wild type DU145 (red bars), or PC3 (blue bars) versus HAEC alone. Genes whose regulation was shared by both endothelial cell types are represented. A significant threshold of a twofold change in gene expression corresponded to p < 0.001. Experiments were performed in duplicate. D Western blot analysis of ANG, CXCL9, EDN1 and TGFB2 protein expression in HUVEC cocultured with DU145 (left side of the panel) or PC3 cells (right side of the panel). Representative images of three experiments