Fig. 6

Regulation of inflammation and immunity genes, and prostate cancer driver genes in PC cells cocultured with endothelial cells. A, B Fold differences of mRNAs of “inflammation & immunity” genes (A) and of “prostate cancer driver” genes (B) between DU145, or PC3, cocultured with HUVEC versus DU145, or PC3, respectively. A significant threshold of a twofold change in gene expression corresponded to p < 0.001. Experiments were performed in duplicate. C Western blot analysis of FASLG, IL1β, IL4 and IL6 protein expression in PC3 (a) and DU145 (b) which were cultured with or without HUVEC. Representative images of three experiments. D, E Fold differences of mRNAs of “inflammation & immunity” genes (D) and of “prostate cancer driver” genes (E) between IL30-DU145 or IL30-PC3 cocultured with HUVEC versus DU145 or PC3, respectively. Results obtained from control EV-transfected cells were comparable to those from wild type cells. A significant threshold of a twofold change in gene expression corresponded to p < 0.001. Experiments were performed in duplicate. F Western blot analysis of EGF, VEGF, LGALS4 and SHBG protein expression in IL30-PC3 (a) and IL30-DU145 (b) which were cultured with or without HUVEC. Representative images of three experiments