Fig. 1
ACTN1 is a critical factor in cisplatin resistance in HNSCC. A Schematic diagram illustrating the PCR array experimental workflow. B Volcano plots representing differentially expressed genes between SCC-1cisR/SCC-23.cisR cells and their respective parental controls. C-D Western blot analysis of ACTN1 expression in HNSCC cells exposed to cisplatin over varying durations. E–F Western blot analysis of ACTN1 expression in HNSCC cells subjected to varying doses of cisplatin for 48 h. G-H qPCR and western blot analyses of ACTN1 expression in cisplatin-resistant HNSCC cells compared to parental control cells. I Western blot analysis of ACTN1 expression in both chemosensitive and chemoresistant HNSCC tissues. J-K Correlation between the staining intensity of ACTN1 in an in-house HNSCC cohort before neoadjuvant chemotherapy and the therapeutic efficacy. L Survival analysis of chemotherapy-treated patients at baseline, stratified by the staining intensity of ACTN1. *P<0.05, **P<0.01, ***P<0.001, ns: not significant