Fig. 4

ITGB4 knockdown suppressed glioma cell migration and proliferation. a-d ITGB4 was knocked down in LN229 and U251 cells. The cell migration and proliferation were analysed by transwell and colony formation assays. Data represent the mean ± SD of three independent experiments. *** p < 0.001 vs. control. e–g LN229 cells with or without ITGB4 knockdown were subcutaneously injected into nude mice (n = 6 in each group) for tumour formation. Representative bright-field imaging of the tumours in the mice implanted the indicated cells. After 5 weeks, mice receiving transplants of the indicated cells were sacrificed. The tumour volume and weight were calculated. ** p < 0.01, *** p < 0.001 vs. control