Fig. 4

CY12-RP2 suppresses the invasion and migration of melanoma cells via blocking Wnt/β-catenin pathway. A The effect of CY12-RP2 on the colony formation of melanoma cell lines. B The colony formation statistical analysis. C Effects of CY12-RP2 on the migratory capacity of melanoma cell lines were analyzed by wound healing assay. The power field scale bar, 100 μm. D Statistical analysis was performed to determine the migrated distance. E Effects of CY12-RP2 on the invasive ability of melanoma cell lines were analyzed by transwell assays. The power field scale bar, 100 μm. F Statistical analysis was performed to determine the number of invaded cells. G The analysis of CY12-RP2 on EMT and apoptosis signaling pathways by using Western blot assay. Data are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 versus control