Fig. 2

Knockdown of RRM2 suppressed cell growth and decreases the migration capability of ATRT cells. A Immunoblotting analyzed RRM2 knockdown efficiency. Endogenous RRM2 expression was inhibited by two independent shRRM2 (shRRM2#1 and shRRM2#2). The parental cells (WT) and luciferase shRNA (shLuc) cells were used as controls. B, C Knockdown RRM2 attenuates cell growth (B) and colony formation abilities (C) in BT12 cell. D, E Wound healing assay (D) and transwell assay (E) shows the efficiency of RRM2 depletion in ATRT cells. For each experiment, the relative values of parental cells and knockdown cells were compared with the shLuc control. Data are presented as the mean ± SD of triplicated independent experiments, ns non-significant, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, Student’s t test