Fig. 3

COH29 showed an anticancer effect on ATRT cells. A IC50 values of COH29 in BT12, Re1P6 and CHLA266 cells. B, C Analysis of cell proliferation (B) and colony formation (C) in COH29-treated cells. D Wound healing assay after cells were treated with COH29 in three days. E Transwell assay analyzed the migration ability of Re1P6 cells after being treated with COH29. A relative rate of cell migration was analyzed from the number of the cells migrating per chamber compare with the control. In all experiments, 0.1% DMSO was treated as the control. Data are presented as the mean ± SD of triplicated independent experiments, ns non-significant, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, Student’s t-test