Fig. 5

RRM2 inhibition induced apoptosis in ATRT cells. A Flow cytometry analysis with Annexin V-PI staining was performed to evaluate the percentage of apoptotic cells. Representative percentage of total apoptotic cells were normalized with control. Data are presented as the means ± SD of three independent experiments. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, Student’s t-test. B, C Immunoblotting for apoptosis markers of BT12 and Re1P6 cells treated with COH29 in 72 h and 48 h, respectively. D HE staining show the size and position of the tumor in the mouse brain. IHC staining detects the expression of cleaved caspase 3 in the ATRT xenograft brain tumor. Scale bar, from left panels to right panels: 1000 µm-100 µm-50 µm