Fig. 2

CircGPRC5A expression in CRC cell lines and correlations between circGPRC5A expression and CRC patients with clinicopathologic features. A FISH was used to investigate the expression of circGPRC5A in paired normal tissues and CRC tissues. B The qRT-PCR was used to confirm the expressions of circGPRC5A in NCM460 and CRC cell lines. C CircGPRC5A expressions in matched normal tissues and 80 CRC tissues. D-F T stage, lymph node status and Tumor size were considered when evaluating the expression of circGPRC5A. G The cellular localizations of circGPRC5A in HCT8 and HT29 cells were investigated using FISH. H-I The qRT-PCR was used to assess the expressions of circGPRC5A in nuclear and cytoplasmic RNA of HCT8 and HT29 cells. Based on data from three independent experiments, values are presented as the mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001