Fig. 4

CircGPRC5A promotes CRC cell invasion, migration, and proliferation in vitro. A CircGPRC5A overexpressions in HCT116 and SW480 were confirmed using qRT-PCR. B–G Following circGPRC5A overexpressions in SW480 and HCT116 cells, CCK-8, colony formation, and Edu assays were used to assess proliferation. H–K Transwell and wound healing assays were used to measure migration and invasion following circGPRC5A overexpressions in SW480 and HCT116 cells. Based on data from three independent experiments, values are presented as the mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001. Following transfection of MS2-CP and circGPRC5A-MS2 into CRC cells, fluorescence and bright field images were obtained