Fig. 8

CircGPRC5A can cause dephosphorylation of YAP-ser127 and YAP-ser109 by stabilizing the PPP1CA protein. A Western blotting was used to examine PPP1CA protein after silencing circGPRC5A. B Phosphoproteomics revealed 4,170 phosphopeptides, 4,793 phosphorylation sites, and 2,390 phosphoproteins. C The pie chart illustrates the types of phosphorylated amino acids from quantitative phosphoproteomics analysis. D Upregulated and downregulated protein phosphorylation levels from quantitative phosphoproteomics analysis. E Bubble chart depicting the deltaP_value and phosphorylation site number. F, G Western blotting was used to examine YAP phosphorylation after silencing and overexpressing PPP1CA or circGPRC5A. H–I The effects of YAP dephosphorylation via PPP1CA on circGPRC5A-induced changes were detected by western blotting