Fig. 4

circATP9A direct interacts with HuR. Notes: A RNA pull-down assay was applied in NSCLC cells to identify the proteins that interacted with circATP9A; B The silver staining image of RNA pull-down with circATP9A probe in A549 cells; C qRT–PCR analysis confirmed that the circATP9A probe could specifically enrich circATP9A in A549 cells by RNA pull-down assay; D Western blot assay in circATP9A pull-down proteins confirmed the interaction between circATP9A2 and HuR; E The subcellular co-localization of circATP9A and HuR in NSCLC cells was measured by fluorescence staining assay; F The catRAPID was used to predict the binding site of circATP9A to HuR; G-H Schematic structures of HuR proteins and 3 truncations of HuR (top); RIP assays confirmed the interaction of truncation 3 of HuR with circATP9A in A549 cells (bottom); I-J Schematic structures of circATP9A and 3 fragments (top); RIP assays confirmed the interaction of fragment 1 of circATP9A with HuR in A549 cells (bottom); K The mRNA expression level of HuR in the circATP9A knockdown and control cells; L The protein expression level of HuR in the circATP9A knockdown and control cells