Fig. 5

NUCKS1 is the target gene of circATP9A/HuR complex. Notes: A Intersection results of correlation analysis and clip-seq data identified 80 molecules; B Expression level of NUCKS1, E2F3, ECT2, EHMT1, SPIN1 and TRIM37 in the circATP9A knockdown and control cells; C Correlation between NUCKS1 and HuR based on TCGA database; D-E RIP assay was performed in A549 and H1299 cells to detect the NUCKS1 expression enriched by IgG and HuR; F-G The expression level of circATP9A, HuR and NUCKS1 in the circATP9A knockdown and control cells; H-I The protein level of NUCKS1 in the circATP9A knockdown and control cells; J-K Western blot assay was performed to measure HuR and NUCKS1 protein level after transfection of HuR siRNA, control siRNA in circATP9A overexpressed NSCLC cells