Fig. 2

4-1BBL is highly expressed in uveal melanoma and is prognostic for worse survival A Tukey Boxplots showing normalized gene expression of TNFSF9 across 33 cancer types of the TCGA-Pan cancer dataset together with a heatmap comparing the relative TIL signature score defined by ssGSEA. The UM cohort (TCGA-UVM) is highlighted as a red boxplot. B Relative TNFSF9 gene expression within the TCGA-UVM cohort comparing tumors with or without loss of chromosome 3. Mann–Whitney was used to test for significance (n = 80). C and D Correlation between TNFSF9 gene expression with (C) BAP1 DNA copy number and (D) BAP1 gene expression. Correlations were analyzed with Spearman’s test. E and F Kaplan–Meier plots of the TCGA-UVM cohort (n = 80) grouped based on the median gene expression of TNFSF9 for (E) overall survival (OS) and (F) Progression-Free survival (PFS). Log-rank test was used to test for hazard ratios and significance. G Tukey Boxplots showing significantly enriched (p < 0.0001) mSigDB hallmarks gene signatures comparing 2 groups of TCGA-UVM tumor cases (n = 80) grouped based on the median gene expression of TNFSF9