Fig. 3

NK cells promote migration of uveal melanoma cells into liver organoids A Representative bright field images of liver tissue-derived organoids visualized at day 3, 6, 13 and 19 under 4X objective. Black and white scale bars denote 650μm and 260μm respectively. B Representative confocal image showing the expression of SOX9 and KRT-19 within liver organoid under 10X objective. White scale bar denotes 40μm. C Bright field image of a liver organoid with pigmented primary UM cells under 4X objective. White scale bar denotes 100μm. D Immunohistochemistry of liver organoid containing a cluster of pigmented UM tumor cells shown from hematoxylin (HTX) staining and 4-1BBL expression via IHC at 4X and 20X objective. Black and white scale bars denote 200μm and 100μm respectively. E Experimental setup of transwell-organoid metastasis in vitro model, representative brightfield image and flow cytometric plot of liver organoid containing mCherry + OCM-1 tumor cells. Scale bar denotes 125μm. F Absolute cell counts of mCherry tumor cells based on flow cytometry of dissociated organoids with or without presence of NK cells. One-way ANOVA with multiple comparison was used to test for significance (n = 5)