Fig. 5

Transcriptomic characterization of sorted CD16⁺ , CD16^- CD103⁺ , CD16^- CD103^- TI-NK cell subsets in treatment naïve breast tumors. Total RNA was extracted from sorted CD16⁺, CD16^-CD103⁺ and CD16^-CD103^- TI-NK cells (CD56⁺ CD3- in DAPI^- CD45⁺ lymphocytes) from breast tumors as well as from CD56^bright CD16^- and CD56^dim CD16⁺ circulating NK cells from paired blood samples and analysed by RNAseq. Data from 3 patients. A Dot and density plot showing sorted TI-NK cell subsets in one representative tumor out of 3 analysed. B Log2 CPM mean expression (z-scores) of DEG in CD56^dim and CD56^bright peripheral blood NK cells, and CD16⁺, CD16^-CD103⁺ and CD16^-CD103^- TI-NK cells. Asterisk label differentially expressed genes in at least one of the three tumor-infiltrating NK cell subsets. C Gene set enrichment analysis (GSEA) of RNAseq data from TI-NK cell subsets against liver and intestine CD56^dim NK/ ILC1/ILC3: GSE37448; NES: normalized enrichment score. FDR: false discovery rate