Fig. 2

AGPS inhibits the proliferation of prostate cancer cells by promoting the promotion of peroxisome production and ferroptosis. a AGPS expression in mRNA after transfection with three shRNAs in DU145 and over expression in 22Rv1 cells. * P < 0.05, ** P < 0.01, *** P < 0.001. b AGPS expression in proteins after transfection with three shRNAs in DU145 and over expression in 22Rv1 cells. c. IC50 values of ML210 and RSL3 in DU145 cells after AGPS knockdown and in 22Rv1 cells after AGPS over expression. ** P < 0.01, *** P < 0.001, **** P < 0.0001. d PMP70 protein expression after AGPS knockdown with shRNA in DU145 cells and over expression in 22Rv1 cells. e PMP70 protein immunofluorescence staining after AGPS knockdown with shRNA in DU145 cells and over expression in 22Rv1 cells. f PCa cells were observed by TEM when induced with ML210 after AGPS knockdown. g MDA levels after AGPS knockdown with shRNA in DU145 cells and over expression in 22Rv1 cells. ** P < 0.01. h CCK8 assay on the OD value in 6-well plates after AGPS knockdown with shRNA in DU145 cells and over expression in 22Rv1 cells. n.s., no significance, * P < 0.05, ** P < 0.01. i and j Colony formation assay on colony numbers of the DU145 cells after AGPS knockdown with shRNA and 22Rv1 cell with AGPS over expression. ** P < 0.01. k PCa cells after AGPS knockdown were injected subcutaneously into the right flank of mice. Xenograft growth was measured every other day for 28 days. Tumors in each group at day 28 were harvested and photographed. l Tumor weight in each group. Data represented as mean ± SD (n = 5). **** P < 0.0001. m Tumor volume at each time point. Data represented as mean ± SD (n = 5). n.s., no significance; *P < 0.01, **P < 0.01, ***P < 0.001