Fig. 10

GABA promotes proliferation and migration of CRC via mir-223-3p/CBLB/cMYC axis. A CCK-8 assays showed that overexpression of CBLB reversed the effect of miR-223-3p on the promotion of proliferation in colon cancer cells. B The colony formation assay indicated that overexpression of CBLB reversed the effect of miR-223-3p on the promotion of proliferation in colon cancer cells. C The transwell assays showed that overexpression of CBLB reversed the effect of miR-223-3p on the promotion of migration in colon cancer cells. D The wound healing assays showed that overexpression of CBLB reversed the effect of miR-223-3p on the promotion of migration in colon cancer cells. E Western blot indicated that overexpression of CBLB reversed the effects of miR-223-3p on the promotion of proliferation and migration in colon cancer cells. F Ubiqutin assays indicated that overexpression of CBLB blocked the altered ubiquitination of cMYC in colon cancer induced by miR-223-3p. All data were revealed as mean ± standard deviation (SD) for no less than three independent experiments. Significant P values showed as ^***P < 0.001.^**P < 0.01