Fig. 2

GABA inhibits ubiquitination of cMYC and promotes proliferation and migration of CRC. A The proliferation of colon cancer cells was assessed via CCK-8 for 3 days. B The proliferation of colon cancer cells was assessed via colony formation assay for 10 days. C, D The wound healing assays showed that GABA significantly augmented the migrative ability of colon cancer cells. E The transwell assays indicated that GABA increased the migrative ability of colon cancer cells. F, H Treatment with GABA increased the volume and weight of subcutaneous tumors. G The solid tumors were peeled off and the weight was measured. H The volume of transplanted tumors was measured by the Vernier caliper per week. I, J The number of tumors in the lung was counted upon treated with GABA or PBS. K HE staining showed the tumors in the lung of mice. All data were revealed as mean ± standard deviation (SD) for no less than three independent experiments. Significant P values showed as ^***P < 0.001.^**P < 0.01