Fig. 3

GABA inhibits ubiquitination of c-MYC and promotes proliferation and migration of CRC. A Colon cancer cells were treated with GABA followed by treatment with cycloheximide (CHX) for the indicated times. The intensity of cMYC expression at each time point was quantified by densitometry and plotted against time. B The expression of cMYC in colon cancer cells treated with GABA for 3 days and then incubated with or without MG132 for 6 h via western blot. C Ubiqutin assays of colon cancer cells treated with GABA and MG132. D The western blot analysis revealed that cMYC knockdown reversed the effects of GABA-induced promotion of proliferation and migration in colon cancer cells. E CCK-8 assays showed that cMYC knockdown reverses the effects of GABA-induced promotion of proliferation in colon cancer cells. F Colony formation assays revealed that cMYC knockdown reverses the effects of GABA-induced promotion of proliferation in colon cancer cells. G Transwell assays revealed that cMYC knockdown reverses the effects of GABA-induced promotion of migration in colon cancer cells. All data were revealed as mean ± standard deviation (SD) for no less than three independent experiments. Significant P values showed as ^***P < 0.001.^**P < 0.01.^*P < 0.05