Fig. 5

Exosome miR-223-3p promotes M2 polarization of macrophages, which aggravates the proliferation and migration of CRC. A A cluster heatmap of the expression profiles of miRNAs in the exosomes derived from PBS-induced LoVo cells and GABA-induced LoVo cells. B The qRT-PCR analysis of miR-223-3p in the CM of LoVo cells was treated with RNase A (3U/μg) alone or combined with Triton X-100 (0.1%) for 20 min. C-D The expression of CD206, CD163 and CD86 in THP1 cells upon miR-223-3p overexpression or not by qRT-PCR and Western blot. E Immunofluorescence assay indicated that miR-223-3p overexpression increased the expression of M2 makers in THP1 cells. F The proliferation of SW480 cells co-cultured with THP1 overexpressed miR-223-3p or not was assessed via CCK-8 for 3 days. G Proliferation of SW480 cells co-cultured with THP1 cells overexpressed miR-223-3p or not was assessed via colony formation assay for 10 days. H The transwell assays indicated that co-cultured with THP1 cells overexpressed miR-223-3p increased the migrative ability of SW480 cells. I The wound healing assays revealed that co-cultured with THP1 cells overexpressed miR-223-3p increased the migrative ability of SW480 cells. All data were revealed as mean ± standard deviation (SD) for no less than three independent experiments. Significant P values showed as ^***P < 0.001.^**P < 0.01.^*P < 0.05. ns means the difference was not significant