Fig. 6

Exosome miR-223-3p promotes M2 polarization of macrophages, which aggravates the proliferation and migration of CRC. A CCK-8 assays showed that proliferation of colon cancer cells was significantly augmented when added the CM of THP1 cells overexpressed miR-223-3p. B The transwell assays indicated that migrative ability was significantly augmented when added the CM of THP1 cells overexpressed miR-223-3p. C The colony formation assay indicated that migrative ability was significantly augmented when added the CM of THP1 cells overexpressed miR-223-3p. D A cluster heatmap of the expression profiles of cytokines in the CM of THP1 cells upon overexpression miR-223-3p or not via ELISA assays. E The expression of cytokines in THP1 cells upon overexpression miR-223-3p or not was detected by qRT-PCR. F Western blot indicated that inhibition of IL17 blocked the altered expression of E-cadherin, N-cadherin, Vimentin, Cyclin E and Cyclin D1 in colon cancer cells co-cultured with THP1 cells overexpressed miR-223-3p. G Western blot showed that the expression of IL17 was conspicuously increased or decreased in THP1 cells upon miR-223-3p overexpression or knockdown in THP1 cells, respectively. H The expression of E-cadherin, Vimentin, Cyclin E and Cyclin D1 in colon cancer cells upon administration of IL17 were detected by Western blot. All data were revealed as mean ± standard deviation (SD) for no less than three independent experiments. Significant P values showed as ^***P < 0.001.^**P < 0.01.^*P < 0.05. ns means the difference was not significant