Fig. 7

GABA promotes the proliferation and migration of CRC by inhibiting ubiquitination of cMYC through miR-223-3p. A Colon cancer cells were overexpressed miR-223-3p followed by treatment with cycloheximide (CHX) for the indicated times. The intensity of cMYC expression at each time point was quantified by densitometry and plotted against time. B Ubiqutin assays of colon cancer cells transfected with miR-mimic followed by treatment with MG132. C. CCK-8 assays revealed that knockdown miR-223-3p reversed the GABA-induced promotion of proliferation in colon cancer cells. D The colony formation assay indicated that knockdown miR-223-3p reversed the GABA-induced promotion of proliferation in colon cancer cells. E The transwell assays showed that knockdown miR-223-3p reversed the GABA-induced strengthened migrative ability of colon cancer cells. F Western blot showed that inhibition of miR-223-3p reversed the GABA-induced promotion of proliferation and migration in colon cancer cells. G Ubiqutin assays indicated that inhibition of miR-223-3p blocked the altered ubiquitination of cMYC in colon cancer induced by GABA. All data were revealed as mean ± standard deviation (SD) for no less than three independent experiments. Significant P values showed as ^***P < 0.001.^**P < 0.01.^*P < 0.05