Fig. 9

GABA promotes proliferation and migration of CRC via mir-223-3p/CBLB/cMYC axis. A CCK-8 assays showed that overexpression of CBLB reversed the effect of GABA on the promotion of proliferation in colon cancer cells. B The colony formation assay indicated that overexpression of CBLB reversed the effect of GABA on the promotion of proliferation in colon cancer cells. C The transwell assays showed that overexpression of CBLB reversed the effect of GABA on the promotion of migration in colon cancer cells. D The wound healing assays showed that overexpression of CBLB reversed the effect of GABA on the promotion of migration in colon cancer cells. E Western blot indicated that overexpression of CBLB reversed the effects of GABA on the promotion of proliferation and migration in colon cancer cells. F Ubiqutin assays indicated that overexpression of CBLB blocked the altered ubiquitination of cMYC in colon cancer induced by GABA. All data were revealed as mean ± standard deviation (SD) for no less than three independent experiments. Significant P values showed as ^***P < 0.001.^**P < 0.01