Fig. 5

Schematic representation of involved variable process for development of multicellular heterotypic 3D breast cancer Organoid. A MCF-7 cells cultured on 2D monolayer. B Early and (C) Late stage of primary mouse embryonic fibroblast. Variable organoid developed by help of Nunclon Sphera ultra-low attachment (D), Poly-HEMA coated (E) and 2% methyl cellulose mediated (F) with a MCF-7 and Fibroblast cell population (80,000 cells and 10:1 cell ratio, 14 days)