Fig. 6

Schematic representation of differential approaches for organoid vascularization. A Templating techniques guide the development of vascular networks in organoids 1) 3D bioprinting utilizes precise 3D Biofabrication to stimulate vascularization within organoids. 2) DMD (Digital Micromirror Device) patterning employs micromirrors for precise modulation light and produces vascularized networks within organoids 3) Using the sacrificial network templating method, temporary structures are developed to direct vascular development in organoids. B Through intrinsic cellular connections, the self-organizing technique promotes spontaneous vascularization inside organoids. 4) Endothelial cell co-culture in compartmentalized microfluidic systems that facilitate in vitro organoid vascularization 5) Use of controlled fluid dynamics in organoids on microfluidic devices to encourage the development of blood vessels