Table 2 Significant mammary organoid study with associated protocol details

1

Organoid cultures from normal and cancer-prone human breast tissues

This investigation looks at the early oncogenic transformation of various breast cancer subtypes using long-term culture of BC organoids. Human mammary epithelial cells (HMECs) from different lineages are cultured according to the specified protocol for an endless period.

[2]

2

Long-term culture, genetic manipulation, and xenotransplantation of human normal and breast cancer organoids

The protocol provides information on the long-term culture of normal and breast cancer organoids, genetic modification, and xenotransplantation for investigating tumorigenesis using patient-relevant cancer drivers and mutations.

[128]

3

Current Status of Breast Organoid Models

The study discusses breast organoids as 3D simulations of the in vivo breast microenvironment and investigates the factors influencing breast cancer development.

[157]

4

A Mammary Organoid Model to Study Branching Morphogenesis

Organoid models made from primary mammary epithelial cells are used in this study to examine the branching morphogenesis of the mammary gland. It seeks to comprehend the signaling cues responsible for promoting branching morphogenesis.

[170]

5

Establishment and long-term culture of mouse mammary stem cell organoids and breast tumor organoids

The protocol explains how to maintain the self-renewal of mammary stem cells (MaSCs) that form glands and how to model tumorigenesis by introducing cancer drivers and mutations relevant to patients.

[171]

6

Organotypic culture assays for murine and human primary and metastatic-site tumors

The study offers valuable suggestions for long-term time-lapse imaging of epithelial morphogenesis in three-dimensional organotypic cultures. It sheds light on how the ECM microenvironment controls the mammary epithelium’s collective migration and dissemination.

[172]

7

BRCA-deficient mouse mammary tumor organoids to study cancer-drug resistance

The study examines cancer drug resistance using BRCA-deficient mouse mammary tumor organoids. The organoids are used to simulate basal-like breast cancer and analyze the role of BRCA2 and p53 as tumor suppressors in breast cancer.

[173]

8

Generation and functional characterization of murine mammary organoids

The protocol details experimental techniques for developing mice mammary organoid lines isolated from mammary glands or tumors caused by mutations in PI3K pathway components. These organoids can monitor drug responses and guide more effective treatments.

[174]