Fig. 1

The enhanced RGCC in lung metastatic TNBC cells. A Schematic diagram to show the organotropic metastasis mouse model establishment of TNBC cells. B Representative bioluminescence images of lung-tropic metastasis of MDA-MB-231 cells in major organs. C Volcano plots of the altered gene expression between lung metastatic cells (LM3) and parental cells (P). The red and green dots represent the significantly upregulated and downregulated RNAs, respectively. D Heatmap shows 20 most upregulated or downregulated genes between MDA-MB-231/LM3 and MDA-MB-231/P cells. E Relative folds of RGCC mRNA expression in various metastatic organ sites were detected by qRT-PCR (P: parental; LM3/HM3/BM3: the third lung/liver/brain metastatic cells.). F Western blot analysis of RGCC protein levels in metastatic 4T1/MDA-MB-231 derived from various organ lesion and parental cells. (The data are presented as the mean ± SD; ^***P < 0.001)