Fig. 5

RGCC stimulates PLK1 kinase activity. A Venn diagram showing the potential interacting proteins of RGCC predicted by the database of Inbio discover, String and Biogrid. B Identification of the potential proteins interacting with RGCC by Mass Spectrometry. C Co-IP assays to confirm the direct interaction between RGCC and PLK1 in MDA-MB-231/LM3 cells using antibodies anti-RGCC or anti-PLK1, respectively. D IF co-staining showing the co-localization of RGCC (Red) and PLK1 (Green) in 4T1/LM3 and MDA-MB-231/LM3 cells. E Co-immunoprecipitation assay was used to detect the direct binding domain using HA-RGCC and FLAG-PLK1 truncation fragments in 293 T cells. F The binding mode of RGCC and PLK1 kinase domain (KD). G Western blot analysis of PLK1 protein levels under RGCC knockdown or overexpression. H PLK1 kinase assay showing RGCC affects PLK1 kinase activity. Data are means ± SD from 3 independent experiments