Fig. 3

RPS7 knockout inhibits HCC cell adhesion, migration and invasion in vitro and metastasis in vivo. MHCC97H and HLE cells, two highly invasive HCC cell lines were used to establish stable RPS7 knockout cells by using CRISPR/Cas9 system (RPS7 KO). Non-target knockout cells (NTC KO) were correspondingly used as controls. The cell-matrix adhesion capacity, migration and invasion ability of cells in vitro as well as metastasis in vivo were observed. A The knockout efficiency was determined by western blot assay. B RPS7-knockout MHCC97H and HLE cells adhesion to fibronectin, collagen I and collagen IV were detected using cell-matrix adhesion assay. C The effect of RPS7-knockut on cell migration and invasion were determined by Transwell assay. D Orthotopic mouse models were constructed using RPS7-knockout MHCC97H cells and control cells (each group, n=12). The effect of RPS7-knockout on tumor size and numbers were evaluated. E and F The effects of RPS7-knockout on lung metastasis were evaluated by orthotopic mouse models and tail vein lung metastasis mouse models, respectively. Representative data are from at least 3 independent experiments. Data are shown as mean ± SD. **, P < 0.01