Fig. 4

LOXL2, a key downstream target of RPS7, is regulated by RPS7 at post-transcriptional level. To elucidate the potential mechanism underlying RPS7-mediated HCC metastasis, RAN-seq analysis was performed using two independent RPS7-knockdown (siRPS7-1 and siRPS7-2) and non-target knockdown (siNTC) MHCC97H cells. A As shown were differentially expressed genes after RPS7 knockdown. B Candidate transcripts that may bind to RPS7 protein were extracted from catRAPID database. C After combing the RNA-seq data and catRAPID data set, differentially expressed genes that possibly bind to RPS7 protein were identified. D KEGG analysis of the candidate downstream genes. E RPS7 knockdown decreased the mRNA and protein levels of LOXL2. F. RPS7 overexpression increased the mRNA and protein levels of LOXL2. G. The effect of RPS7 on LOXL2 mRNA half-life was determined by RNA decay assay. **, P < 0.01