Fig. 6

LOXL2 promotes focal adhesion (FA) formation, migration and invasion of HCC cells. A and B To evaluate the role of LOXL2 in HCC metastasis, two pcDNA3.1-LOXL2-3×Flag mutant plasmids, catalytic activity deletion mutant of LOXL2 (LOXL2-Δ) and catalytically inactive point mutant of LOXL2 (LOXL2-Y689F), as well as wild type LOXL2 (LOXL2-WT) were respectively constructed and stably transfected into Huh7 cells. The effect of LOXL2 overexpression on cell-matrix adhesion ability (A) and migration and invasion (B) were analyzed. C The effect of LOXL2 silencing on cell-matrix adhesion ability in MHCC97H cells. D The effect of LOXL2 silencing on cell migration and invasion in MHCC97H cells. E-G. The effect of LOXL2 silencing (E), 20 μM CMMH (F), and LOXL2 overexpression (G) on FA formation in HCC cells were respectively detected by Immunofluorescence staining experiments. Representative data are from at least 3 independent experiments. Data are shown as mean ± SD. **, P < 0.01. ns, no significant