Fig. 7

LOXL2 up-regulates ITGB1 via enhancing its protein stability. FA is a well-characterised structure closely regulating tumor cell adhesion and migration, and is consist of numerous proteins. The molecular mechanism underlying the role of LOXL2 in FA formation was further explored. A Western blot assay was used to assess the influence of LOXL2 on the expression of several key structure proteins of FA. B Protein half-life measurements based on CHX treatment were performed to evaluate the impact of LOXL2 on the stability of ITGB1 protein. C Whether LOXL2 regulated ITGB1 protein degradation through the proteasome degradation pathway was determined by western blotting analysis. Cells were treated with 10 μmol/L MG132 for 12 h. D Western blotting analysis was performed to determine whether LOXL2 regulated degradation of ITGB1 by proteasome pathway depending on ubiquitin. Cells were treated with 20 μmol/L PYR-41 for 20 h. E Whole-cell extracts of LOXL2 knockdown MHCC97H cells were immunoprecipitated with anti-ITGB1 antibody, and ubiquitinated ITGB1 was detected with anti-ubiquitin antibody. F A reciprocal co-IP assay was performed in HLE cells. G The effect of LOXL2 on signal activity of FAK/SRC pathway in MHCC97H cells was determined by western blotting