Fig. 2

Treatment with MS-275 impacts on EOC 3D spheroid generation and on spheroid mediated peritoneal clearance (A) OVCAR-3 cells 3D spheroids were generated in the presence of MS-275 (1 μM). B SKOV3 cells 3D spheroids were generated in the presence of MS-275 (2.5 μM). Images were taken at 0, 24, 48 and 96 hours. Quantification of the area is shown at the right of the images. Cell vitality at 96 hours was analyzed by staining with Calcein AM. Representative images are shown from one experiment of 4 performed. C Images show mesothelial clearance induced by OVCAR-3 spheroids treated or not with MS-275 (1 μM) after 4 and 24 hours. The chart at the right of the images represents the ratio in percentage between the area of ​​the gap formed by the spheroid on the mesothelial monolayer (time 24 hours, labeled in yellow) and the spheroid area (time 0 hours). Representative images are shown from one experiment of 6 performed. Differences were considered significant at P < 0.05 (*p < 0.05; **p < 0.01; ***p < 0.001)