Fig. 7

Talin-1 ectopic expression rescues the altered Actin polymerization, FN-1 extracellular distribution and MC/EOC adhesion upon treatment with MS-275 (A) A human control peritoneum shows a conserved MC monolayer negative for FN-1 (upper left). FN-1 (arrows) strongly stains the tumor surrounding stroma in a sample from a peritoneal carcinomatosis patient (upper right). The MC monolayer of a control peritoneal sample shows low levels of Talin-1 expression (bottom left). CAFs and tumor nodules accumulated in the sub-mesothelial compact zone of a patient sample show high staining for Talin-1 (bottom right). Scale bars: 50 𝜇m. MCs: Mesothelial cells; CAFs: carcinoma-associated fibroblasts; T: tumor. B Western blot showing ectopic expression of Talin-1 in Met5A cells treated with MS-275 (250 nM) for 72 hours. HSP90 was used as a loading control. One of three experiments is shown. Quantification of the experiment is shown on the right. C Immunofluorescence of Met5A cells treated with MS-275 (250 nM) for 72 hours where Talin-1 was ectopically expressed. Phalloidin staining to mark Actin (red) is shown in the top, FN-1 staining (green) is shown in the bottom. Nuclei were stained with DAPI. Representative images are shown from one of three experiments performed. D Adhesion assays on MeT5A cells where Talin-1 was ectopically expressed. SKOV3 cells were stained with PHK26. Results are shown as the relative number of adherent SKOV3 cells on Talin-1 ectopically expressed MeT5A monolayers. Adherent SKOV3 cells were evaluated in 3 fields/sample. Bars represent means±SEM of 3 experiments. Differences were considered significant at P < 0.05 (*p < 0.05; **p < 0.01; ***p < 0.001; **** p < 0.0001)